Engineering of vitamin prototrophy in Clostridium ljungdahlii and Clostridium autoethanogenum
نویسندگان
چکیده
منابع مشابه
Lactose-inducible system for metabolic engineering of Clostridium ljungdahlii.
The development of tools for genetic manipulation of Clostridium ljungdahlii has increased its attractiveness as a chassis for autotrophic production of organic commodities and biofuels from syngas and microbial electrosynthesis and established it as a model organism for the study of the basic physiology of acetogenesis. In an attempt to expand the genetic toolbox for C. ljungdahlii, the possib...
متن کاملH2 drives metabolic rearrangements in gas-fermenting Clostridium autoethanogenum
Background The global demand for affordable carbon has never been stronger, and there is an imperative in many industrial processes to use waste streams to make products. Gas-fermenting acetogens offer a potential solution and several commercial gas fermentation plants are currently under construction. As energy limits acetogen metabolism, supply of H2 should diminish substrate loss to CO2 and ...
متن کاملGenome editing of Clostridium autoethanogenum using CRISPR/Cas9
BACKGROUND Impactful greenhouse gas emissions abatement can now be achieved through gas fermentation using acetogenic microbes for the production of low-carbon fuels and chemicals. However, compared to traditional hosts like Escherichia coli or yeast, only basic genetic tools exist for gas-fermenting acetogens. To advance the process, a robust genetic engineering platform for acetogens is essen...
متن کاملEffect of Organic Substrate on Promoting Solventogenesis in Ethanologenic Acetogene Clostridium ljungdahlii ATCC5538
Clostridium ljungdahlii is a strictly anaerobic acetogene known for its ability to ferment a wide variety of substrates to ethanol and acetate. This bacterium presents a complex anaerobic metabolism including the acetogenic and solventogenic phases. In this study, the effect of various carbon sources on triggering the metabolic shift toward solventogenesis was considered. The bacterium was grow...
متن کاملExpanding the molecular toolkit for the homoacetogen Clostridium ljungdahlii
Increasing interest in homoacetogenic bacteria for the production of biochemicals and biofuels requisites the development of new genetic tools for these atypical production organisms. An attractive host for the conversion of synthesis gas or electricity into multi-carbon compounds is Clostridium ljungdahlii. So far only limited achievements in modifying this organism towards the production of i...
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ژورنال
عنوان ژورنال: Applied Microbiology and Biotechnology
سال: 2019
ISSN: 0175-7598,1432-0614
DOI: 10.1007/s00253-019-09763-6